3 ports Fluorescence Mini Cube - Single Fluorophore
3 ports Fluorescence Mini Cube - Single Fluorophore
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Single excitation band fiber photometry measurements use an FMC with 3 ports: one port for the excitation light, one for the fluorescence detection, and one to connect to the sample. The cube has a dichroic mirror to let the excitation light through and separate the fluorescence emission and narrow bandpass filters to limit the excitation or fluorescence spectrum. E stands for fluorescence excitation, F for fluorescence, and S for the sample port on the cube's schematic. Currently, only Green Fluorophore (GFP-like) configurations or Red Fluorophore (RFP-like) are offered. Still, the filter set can be customized for other fluorophores on request.
The Intensity Adjustment Ring on the 460-490 nm LED light source reduces the source power below the LED driver's range if needed for low-power fiber photometry experiments.
Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, the versions where LEDs are integrated as well reduce the number of optical connections and the system’s overall footprint.
| Mini Cube | |
|---|---|
| Wavelength range | 350 to 1100 nm |
| Optical fiber compatibility |
- Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth |
Green Fluorophore : - Excitation 460-490 nm - Emission 500-550 nm Red Fluorophore : - Excitation 540-570 nm - Emission 580-680 nm |
| Optical filter attenuation | > OD 5 outside band |
| Dimensions | 57 mm x 63 mm x 26 mm |
| Built-in Detectors | |
| Detector Sensitivity | - 0.38 A/W @ 550 nm - 0.60 A/W @ 960 nm (peak) |
| Amplification | 3 levels : 1x, 10x, 100x |
| Transimpedance gain |
- 2.0 E09 V / A (1x) - 2.0 E10 V / A (10x) - 2.0 E11 V / A (100x) |
| Conversion gain @ 550 nm |
- 0.76 V / nW (1x) - 7.60 V / nW (10x) - 76.0 V / nW (100x) |
| Saturation level @ 550 nm |
- 7.2 nW (1x) - 0.72 nW (10x) - 72 pW (100x) |
| NEP | < 12 fW/√Hz |
| Bandwidth | - DC : 0 - 1000 Hz - AC : 30 - 1000 Hz |
| Electrical interface | BNC |
| Output Voltage | 0.0 to 5.5 V |
| Output impedance | 50 Ω |
| Sensor type | Silicon photodiode |
| Sensor Area | 1 x 1 mm |
| Input voltage | 12 V DC (power supply included) |
| Built-in LEDs (ilFMC Gen.2) |
|
| Max Current | 500 mA |
| Emitter size | 400 µm diameter - NA0.22 |
| Max Output Power (fibre 400 µm) |
LED 460-490 nm = 1.5 to 120 µW (adjustable) |
| LED 540-570 nm = 50 µW (fixed) |
|
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Single excitation band fiber photometry measurements use an FMC with 3 ports: one port for the excitation light, one for the fluorescence detection, and one to connect to the sample. The cube has a dichroic mirror to let the excitation light through and separate the fluorescence emission and narrow bandpass filters to limit the excitation or fluorescence spectrum. E stands for fluorescence excitation, F for fluorescence, and S for the sample port on the cube's schematic. Currently, only Green Fluorophore (GFP-like) configurations or Red Fluorophore (RFP-like) are offered. Still, the filter set can be customized for other fluorophores on request.
The Intensity Adjustment Ring on the 460-490 nm LED light source reduces the source power below the LED driver's range if needed for low-power fiber photometry experiments.
Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, the versions where LEDs are integrated as well reduce the number of optical connections and the system’s overall footprint.
-
Mini Cube Wavelength range 350 to 1100 nm Optical fiber compatibility - Core diameter 200 or 400 µm
- NA 0.37 to 0.57Spectral bandwidth Green Fluorophore :
- Excitation 460-490 nm
- Emission 500-550 nm
Red Fluorophore :
- Excitation 540-570 nm
- Emission 580-680 nmOptical filter attenuation > OD 5 outside band Dimensions 57 mm x 63 mm x 26 mm Built-in Detectors Detector Sensitivity - 0.38 A/W @ 550 nm
- 0.60 A/W @ 960 nm (peak)Amplification 3 levels : 1x, 10x, 100x Transimpedance gain - 2.0 E09 V / A (1x)
- 2.0 E10 V / A (10x)
- 2.0 E11 V / A (100x)Conversion gain
@ 550 nm- 0.76 V / nW (1x)
- 7.60 V / nW (10x)
- 76.0 V / nW (100x)Saturation level
@ 550 nm- 7.2 nW (1x)
- 0.72 nW (10x)
- 72 pW (100x)NEP < 12 fW/√Hz Bandwidth - DC : 0 - 1000 Hz
- AC : 30 - 1000 HzElectrical interface BNC Output Voltage 0.0 to 5.5 V Output impedance 50 Ω Sensor type Silicon photodiode Sensor Area 1 x 1 mm Input voltage 12 V DC (power supply included) Built-in LEDs (ilFMC Gen.2) Max Current 500 mA Emitter size 400 µm diameter - NA0.22 Max Output Power
(fibre 400 µm)LED 460-490 nm
= 1.5 to 120 µW (adjustable)LED 540-570 nm
= 50 µW (fixed)