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The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

Notes

  • The electrical and optical fiber tethers of an efocus Fluorescence Microscope Body Gen 3 are the same length.
  • Every microscope body comes with a protective cap.
  • The efocus Fluorescence Microscope Body Gen 3 is only compatible with the Twist-on efocus Imaging Cannulas.
   Field of View  650 µm x 650 µm
   Working distance adjustment range 0 - 300 µm
   Frame rate > 47 fps
   Dimensions  10 mm x 15 mm x 22 mm
   Mass  3 g
   Computer specifications  - Intel Core-i7
 - 16GB RAM
 - Gigabit Ethernet, Jumbo frame compatible
 - Dedicated graphic card
Fluorescence recording
   Green fluorescence bodies - Excitation 458 / 35 nm
- Emission 525 / 40 nm
   Red fluorescence bodies - Excitation 540 / 15 nm
- Emission 609 / 57 nm
Optogenetic stimulation*
   Green fluorescence bodies - spectral bandwith 612 / 69 nm
- activation intensity 0 to 55 mW/mm2
   Red fluorescence bodies - spectral bandwith 465 / 20 nm
*Fluorescence + optogenetic microscopes only
Drawing

efocus Fluorescence Microscope Body

User ManualFrEn

efocus Fluorescence Microscope System Gen 3

Application NoteFrEn

GCaMP configuration filter set

RCaMP2 configuration filter set

GCaMP-NpHR3 configuration filter set

GCaMP-ChR2 configuration filter set

 

  • The 3rd generation efocus Fluorescence Microscope enables calcium imaging with cellular resolution over a larger brain area of freely-moving behaving animals. Its field of view has increased to 650 x 650 µm, and the electronic depth adjustment is 300 µm. A more sensitive sensor of the 3rd generation microscope requires less fluorescence excitation light, reducing photobleaching and phototoxicity. The microscope body attaches to the imaging cannula with a simple barrel rotation. No tools are required.

    Notes

    • The electrical and optical fiber tethers of an efocus Fluorescence Microscope Body Gen 3 are the same length.
    • Every microscope body comes with a protective cap.
    • The efocus Fluorescence Microscope Body Gen 3 is only compatible with the Twist-on efocus Imaging Cannulas.
  •    Field of View  650 µm x 650 µm
       Working distance adjustment range 0 - 300 µm
       Frame rate > 47 fps
       Dimensions  10 mm x 15 mm x 22 mm
       Mass  3 g
       Computer specifications  - Intel Core-i7
     - 16GB RAM
     - Gigabit Ethernet, Jumbo frame compatible
     - Dedicated graphic card
    Fluorescence recording
       Green fluorescence bodies - Excitation 458 / 35 nm
    - Emission 525 / 40 nm
       Red fluorescence bodies - Excitation 540 / 15 nm
    - Emission 609 / 57 nm
    Optogenetic stimulation*
       Green fluorescence bodies - spectral bandwith 612 / 69 nm
    - activation intensity 0 to 55 mW/mm2
       Red fluorescence bodies - spectral bandwith 465 / 20 nm
    *Fluorescence + optogenetic microscopes only
  • Drawing

    efocus Fluorescence Microscope Body

    User ManualFrEn

    efocus Fluorescence Microscope System Gen 3

    Application NoteFrEn

    GCaMP configuration filter set

    RCaMP2 configuration filter set

    GCaMP-NpHR3 configuration filter set

    GCaMP-ChR2 configuration filter set