Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP, Yellow Optogenetics (OBSOLETE)

Rotary Bundle-imaging Fluorescence Mini Cubes - GCaMP, Yellow Optogenetics (OBSOLETE)

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This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with yellow optogenetic inhibition. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.

   Wavelength range   350 to 1100 nm
   Field of view   Ø1.25 mm
   Objective NA   0.50
  Maximum number of sites
  * could be limited by patchcord manufacturing		 - 7x core 400 µm NA0.57
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37
   Excitation Uniformity   10% over FOV
   Optical fiber compatibility - Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57
   Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm
 - Excitation #2 : 460-490 nm
- Emission #1 : 500-550 nm
- Opto #1 : 580-650 nm
   Optical filter attenuation > OD 5 outside band
   Optical fiber connector  SMA
   Dimensions  92 mm x 83 mm x 267mm
SENSOR
   Type   CMOS Image sensor
   Resolution
   540 x 540 px
   Pixel Size
   6.9 µm x 6.9 µm
   Frame rate
   up to 60 Hz (1 excitation)
  up to 30 Hz (2 excitations)
   Computer interface   USB 2.0 
Drawing

Rotary Bundle-imaging Fluorescence Mini Cubes
User ManualFrEn

Rotary Bundle-imaging Fluorescence Mini Cubes
Brochure

Rotary Bundle Photometry System

  • This Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC) combines Isosbestic & Functional GCaMP excitations with yellow optogenetic inhibition. Isosbestic and functional fluorescence signals are recorded with the same image sensor by interleaving the excitation signals. Rotary bundle-imaging enables simultaneous recordings from all sites within Ø1.25 mm field of view, limiting the applicable bundle diameter. Consider the non-rotating Bundle-imaging Fiber Photometry Systems for a larger field of view.

  •    Wavelength range   350 to 1100 nm
       Field of view   Ø1.25 mm
       Objective NA   0.50
      Maximum number of sites
      * could be limited by patchcord manufacturing    		 - 7x core 400 µm NA0.57
    - 20x core 200 µm NA0.57
    - 90x core 100 µm NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 100, 200, or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm
     - Excitation #2 : 460-490 nm
    - Emission #1 : 500-550 nm
    - Opto #1 : 580-650 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector  SMA
       Dimensions  92 mm x 83 mm x 267mm
    SENSOR
       Type   CMOS Image sensor
       Resolution
    		   540 x 540 px
       Pixel Size
    		   6.9 µm x 6.9 µm
       Frame rate
    		   up to 60 Hz (1 excitation)
      up to 30 Hz (2 excitations)
       Computer interface   USB 2.0 
  • Drawing

    Rotary Bundle-imaging Fluorescence Mini Cubes
    User ManualFrEn

    Rotary Bundle-imaging Fluorescence Mini Cubes
    Brochure

    Rotary Bundle Photometry System