Acute Single-cell Oephys Recording System (OBSOLETE)
Acute Single-cell Oephys Recording System (OBSOLETE)
To better understand the functioning of the brain, it is necessary to precisely understand the activity of neurons and their network.
Using extracellular in vivo electrophysiological recordings, it is possible to monitor neuronal activity from outside the cell. It provides a means to measure patterns of action potentials within many areas of the nervous systems.
Since the extracellular recording are not neuron specific, we developed two kinds of systems where electrical recording is combined with two different techniques (Optogenetics and photometry).
In both Optogenetics and photometry cells are labelled with specific fluorophores, thus providing insights at the cellular level.
Single-cell Optical and Electrical Recordings
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To better understand the functioning of the brain, it is necessary to precisely understand the activity of neurons and their network.
Using extracellular in vivo electrophysiological recordings, it is possible to monitor neuronal activity from outside the cell. It provides a means to measure patterns of action potentials within many areas of the nervous systems.
Since the extracellular recording are not neuron specific, we developed two kinds of systems where electrical recording is combined with two different techniques (Optogenetics and photometry).
In both Optogenetics and photometry cells are labelled with specific fluorophores, thus providing insights at the cellular level. -
Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration. It is designed to activate optogenetic proteins such as channelrhodopsine or to inhibit light-gated ion pumps like halorhodopsin. By adjusting the illumination output power, it is possible to illuminate a single cell or a group of neurons. Extracellular electrophysiology is possible by filling the fiber probe hollow core with an electrolyte solution to get an electrode impedance in the range of 1 to 20 M, allowing a single-unit or multi-unit spikes detection.
See Recording the Illuminated Neuron animation from Biomedical Communications on Vimeo.
This system includes specifically:- Connectorized LED (1x or 2x)
- LED Driver
- Single-cell Optoelectric Probe Holder
- Clamp for Single-cell Optoelectric Probe Holder
- Single-cell Optoelectric Probe Adapter
- Single-cell Optoelectric Probe Interconnect Wire (5x)
- Single-cell Optoelectric Probe Tips (20x)
- Extracellular Electrophysiology Recording System (under development)
- All electrical cables
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This system is designed to do extracellular electrophysiology recordings and photometry detection at a single-cell resolution in a head-fixed configuration. Its optical sensitivity allows detection of standard fluorophores (GFP, mCherry, quantum dot, etc.) or functional fluorophores (GCaMP, Oregon Green, FURA-2, etc). Extracellular electrophysiology is possible by filling the fiber probe hollow core with an electrolyte solution to get an electrode impedance in the range of 1 to 20 M, allowing a single-unit or multi-unit spikes detection.
See Recording the Illuminated Neuron animation from Biomedical Communications on Vimeo.
This system includes specifically:- Fiber Photometry Console
- Connectorized Fluorescence Mini Cube (single or multiple wavelengths with appropriate filters)
- Photosensor Module (1 or 2x)
- Power Supply for PMT Module (1 or 2x)
- Laser Diode Fiber Light Sources (1 or 2x)
- Single-cell Optoelectric Probe Holder
- Clamp for Single-cell Optoelectric Probe Holder
- Single-cell Optoelectric Probe Adapter
- Single-cell Optoelectric Probe Interconnect Wire (5x)
- Single-cell Optoelectric Probe Tips (20x)
- Extracellular Electrophysiology Recording System (under development)
- All electrical cables and optical patch cords
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Single-cell Optical and Electrical Recordings
- Application Note