Basic Fiber Photometry System
Basic Fiber Photometry System
The conventional fiber photometry systems for behaving animals consist of Fiber Photometry Console (FPC), an integrated Fluorescence Mini Cube (FMC), Pigtailed 1x1 Fiber-optic Rotary Joint (FRJ_1x1_pt), and Mono Fiber-optic Cannula (MFC). The fiber-optic rotary joint transfers the light signals and allows fiber tether rotation. Currently, we offer LED light sources for GCaMP (or other green fluorophores), GCaMP with Red Fluo, and FRET(CFP/YFP). Explore the possibility of customization with other light sources, fluorophores, or assisted rotary joint.
The GCaMP Fiber Photometry System records calcium-independent GCaMP fluorescence excited by 405, 415, 425, or 440 nm light (isosbestic point), and its calcium-dependent fluorescence excited by 470 nm light with a single photodetector. Those fluorescence emissions can be demodulated by lock-in amplifier detection or by interleaved excitations.
The GCaMP Fiber Photometry System includes:
- Neuroscience Console 500, the core of the system
- Fluorescence Mini Cube - GCaMP with built-in LEDs, detector and driver
-
Doric Neuroscience Studio software
- All necessary cables and an output fiber optic patch cord
Optional items:
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with pre-assembled parts
- Behavior Camera or CamLoop to record animal behavior
-
Optical Power Meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
-
Low-autofluorescence Optical Patch Cords bring light to an implanted cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule-to-ferrule interconnect
Note: Other light sources and fluorophore combinations are possible.
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The conventional fiber photometry systems for behaving animals consist of Fiber Photometry Console (FPC), an integrated Fluorescence Mini Cube (FMC), Pigtailed 1x1 Fiber-optic Rotary Joint (FRJ_1x1_pt), and Mono Fiber-optic Cannula (MFC). The fiber-optic rotary joint transfers the light signals and allows fiber tether rotation. Currently, we offer LED light sources for GCaMP (or other green fluorophores), GCaMP with Red Fluo, and FRET(CFP/YFP). Explore the possibility of customization with other light sources, fluorophores, or assisted rotary joint.
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The GCaMP Fiber Photometry System records calcium-independent GCaMP fluorescence excited by 405, 415, 425, or 440 nm light (isosbestic point), and its calcium-dependent fluorescence excited by 470 nm light with a single photodetector. Those fluorescence emissions can be demodulated by lock-in amplifier detection or by interleaved excitations.
The GCaMP Fiber Photometry System includes:
- Neuroscience Console 500, the core of the system
- Fluorescence Mini Cube - GCaMP with built-in LEDs, detector and driver
-
Doric Neuroscience Studio software
- All necessary cables and an output fiber optic patch cord
Optional items:
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with pre-assembled parts
- Behavior Camera or CamLoop to record animal behavior
-
Optical Power Meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
-
Low-autofluorescence Optical Patch Cords bring light to an implanted cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule-to-ferrule interconnect
Note: Other light sources and fluorophore combinations are possible. -
The GCaMP with Red Opsin Fiber Photometry System measures calcium-independent GCaMP fluorescence excited by 405, 415, 425, or 440 nm light (isosbestic point), and its calcium-dependent fluorescence excited by 470 nm light with a single photodetector. Those fluorescence emissions can be demodulated by lock-in amplifier detection or by interleaved excitations. It also features an optical fiber input port to connect to red opsin activation or inhibition light source.
The GCaMP with red opsin Fiber Photometry System includes:
- Neuroscience Console 500, the core of the system
-
LD Fiber Light Source for opsin excitation at 638 nm
OR
★LISER™ Light Source for opsin excitation between 580 nm and 680 nm
- Fluorescence Mini Cube - GCaMP with red opsin with built-in LEDs, detector and driver
- Doric Neuroscience Studio software
- All necessary cables and fiber optic patch cord
Optional items:
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with pre-assembled parts
- Behavior Camera or CamLoop to record animal behavior
-
Optical Power Meter to monitor and calibrate excitation power
- Workstation
-
danse™ analyzing software
Accessories:
-
Low-autofluorescence Optical Patch Cords to bring light to the cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
Note: Other light sources and fluorophore combinations are possible. -
The GCaMP & Red Fluophore Fiber Photometry System measures calcium-independent GCaMP fluorescence excited by 405, 415, 425, or 440 nm light (isosbestic point), and its calcium-dependent fluorescence excited by 470 nm light with a single photodetector. A second detector records the fluorescence of Red fluorophores excited with a 560 nm LED. The calcium-dependent GCaMP's fluorescence emissions are demodulated from its calcium-independent emissions by lock-in amplifier detection or by interleaved excitations.
The GCaMP with red fluorophore Fiber Photometry System includes :
- Neuroscience Console 500, the core of the system
- Fluorescence Mini Cube - GCaMP with red fluorophore with built-in LEDs, detector and driver
- Doric Neuroscience Studio software
- All necessary cables and an output fiber optic patch cord
Optional items :
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with pre-assembled parts
- Behavior Camera or CamLoop to record animal behavior
-
Optical Power Meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
-
Low-autofluorescence Optical Patch Cords to bring light to the cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
Note: Other light sources and fluorophore combinations are possible. -
The GCaMP & Red Fluophore with Red Opsin Fiber Photometry System measures calcium-independent GCaMP fluorescence excited by 405, 415, 425, or 440 nm light (isosbestic point), and its calcium-dependent fluorescence excited by 470 nm light with a single photodetector. A second detector records the fluorescence of red fluorophores excited with a 560 nm LED. The calcium-dependent GCaMP's fluorescence emissions are demodulated from its calcium-independent emissions by lock-in amplifier detection or by interleaved excitations. It also features an optical fiber input port to connect to red opsin activation or inhibition light source.
The GCaMP with red fluo and red opsin Fiber Photometry System includes:
- Neuroscience Console 500, the core of the system
- LD Fiber Light Source to control and emit opsin LED power
- Fluorescence Mini Cube - GCaMP with red fluo and red opsin with built-in LEDs, detector and driver
- Doric Neuroscience Studio software
- All necessary cables and fiber optic patch cord
Optional items:
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
- Pigtailed 2x2 Fiber-optic Rotary Joint with Holder
- Fiber Photometry Cannula Holder to record during surgery
- Fiber Photometry Rack with pre-assembled parts
- Behavior Camera or CamLoop to record animal behavior
-
Optical Power Meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
-
Low-autofluorescence optical Patch Cords to bring light to the cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule to ferrule interconnect
Note: Other light sources and fluorophore combinations are possible.