Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

Rotary Bundle-imaging Fluorescence Mini Cubes Gen.2 - Cube

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Mini Cube of the Rotary Bundle-imaging fiber photometry (RBFMC-G2) system contain all the LEDs, CMOS camera, dichroic mirrors and filters required for photometry excitation and detection. A core feature of these mini cubes is they can be fixed at the bottom of the RBFMC-G2 providing signal detection during the rotation and completely abolishing motion artifacts. In addition, different configurations of mini cubes can be swapped on the same RBFMC-G2 base. Note that additional mini cubes are sold separately.

Mini Cube Configurations:
1. Green and red photometry
2. Green photometry and red optogenetics
*Custom cube can be made upon request.

GCaMP + RFP GCaMP + Red Opto
   Wavelength range   350 to 1100 nm
   Field of view   Ø1.30 mm
   Objective NA   0.50
  Maximum number of sites
  * could be limited by patchcord manufacturing		 - 7x core 400 µm NA0.57
- 20x core 200 µm NA0.57
- 90x core 100 µm NA0.37
   Excitation Uniformity   10% over FOV
   Optical fiber compatibility - Core diameter 100, 200, or 400 µm
- NA 0.37 to 0.57
   Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
 - Excitation #2 : 460-490 nm
- Excitation #3 : 555-570 nm
- Emission #1 : 500-550 nm
- Emission #2 : 580-680n
 - Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
- Excitation #2 : 460-490 nm
- Excitation #3 : 580-650 nm
- Emission #1 : 500-540 nm
   Optical filter attenuation > OD 5 outside band
   Optical fiber connector  SMA
   Dimensions  102 mm x 102 mm x 346mm
SENSOR
   Type   CMOS Image sensor
   Resolution
   540 x 540 px
   Pixel Size
   6.9 µm x 6.9 µm
   Frame rate
   up to 60 Hz (1 excitation)
  up to 30 Hz (2 excitations)
   Computer interface   USB 3.0
Build-in LEDs
   Max Current   500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
  1000 mA (LED 555-570 nm)
   1000 mA (LED Opto 600-640 nm)
   Max Output Power 
(400 µm fiber, NA 0.57)		  LED 400-410 nm or 410-415 nm = 60 µW (at 200 mA, CW)
 LED 460-490 nm = 230 µW (at 200 mA, CW)
 LED 555-570 nm = 150 µW (at 200 mA, CW)
  LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
Additional Optogenetic site
  Connector   M8
  Max Current 1000 mA
  Electrophysiology
  Connector  PZN12
Drawing

Rotary Bundle-imaging Fluorescence Mini Cubes - Cube
  • Mini Cube of the Rotary Bundle-imaging fiber photometry (RBFMC-G2) system contain all the LEDs, CMOS camera, dichroic mirrors and filters required for photometry excitation and detection. A core feature of these mini cubes is they can be fixed at the bottom of the RBFMC-G2 providing signal detection during the rotation and completely abolishing motion artifacts. In addition, different configurations of mini cubes can be swapped on the same RBFMC-G2 base. Note that additional mini cubes are sold separately.

    Mini Cube Configurations:
    1. Green and red photometry
    2. Green photometry and red optogenetics
    *Custom cube can be made upon request.

  • GCaMP + RFP GCaMP + Red Opto
       Wavelength range   350 to 1100 nm
       Field of view   Ø1.30 mm
       Objective NA   0.50
      Maximum number of sites
      * could be limited by patchcord manufacturing    		 - 7x core 400 µm NA0.57
    - 20x core 200 µm NA0.57
    - 90x core 100 µm NA0.37
       Excitation Uniformity   10% over FOV
       Optical fiber compatibility - Core diameter 100, 200, or 400 µm
    - NA 0.37 to 0.57
       Spectral bandwidth - Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
     - Excitation #2 : 460-490 nm
    - Excitation #3 : 555-570 nm
    - Emission #1 : 500-550 nm
    - Emission #2 : 580-680n
    		 - Excitation #1 : 405-410 or 410-415 nm or 421-433 nm or 433-445 nm
    - Excitation #2 : 460-490 nm
    - Excitation #3 : 580-650 nm
    - Emission #1 : 500-540 nm
       Optical filter attenuation > OD 5 outside band
       Optical fiber connector  SMA
       Dimensions  102 mm x 102 mm x 346mm
    SENSOR
       Type   CMOS Image sensor
       Resolution
    		   540 x 540 px
       Pixel Size
    		   6.9 µm x 6.9 µm
       Frame rate
    		   up to 60 Hz (1 excitation)
      up to 30 Hz (2 excitations)
       Computer interface   USB 3.0
    Build-in LEDs
       Max Current   500 mA (LED 400-410 nm or 410-415 nm or 421-433 nm or 433-445 nm, LED 460-490 nm)
      1000 mA (LED 555-570 nm)
    		   1000 mA (LED Opto 600-640 nm)
       Max Output Power 
    (400 µm fiber, NA 0.57)    		  LED 400-410 nm or 410-415 nm = 60 µW (at 200 mA, CW)
     LED 460-490 nm = 230 µW (at 200 mA, CW)
     LED 555-570 nm = 150 µW (at 200 mA, CW)
    		  LED Opto 600-640 nm = 7 mW (at 1000 mA, CW)
    Additional Optogenetic site
      Connector   M8
      Max Current 1000 mA
      Electrophysiology
      Connector  PZN12
  • Drawing

    Rotary Bundle-imaging Fluorescence Mini Cubes - Cube