Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics

Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics

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The Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics (BFTO) enables multi-site measurements through High-density Fiber-optic Patch Cords (HDP). These patch cords are available in either square or hexagonal patterns of 7, 9 or 19 fibers connected to the sample port, allowing combined optogenetics and photometry recording.


Build-in LEDs combine Isosbestic & Functional GCaMP excitations with Red Fluorophore excitation. Isosbestic and functional fluorescent signals are recorded with the same image sensor by interleaving the excitations. Both the LED driver and the CMOS detector are integrated within the system. The optogenetics stimulation pattern can vary in type, number and emitted wavelengths. For this purpose, a second similar high-density fiber optic patch cord is connected to the optogenetic port and fans out into separate connectors. Then, up to 19 independent optogenetic stimulation inputs can be connected depending on the needs of the experiment (one input per fiber connected to the sample port).
The BFTO system with targeted optogenetics supports the following fluorophore and opsin combinations:

 


 Fluorophores

Green Fluorophore 
(GCAMP, dLight)

Exc 460-490nm / Em 500-540nm

Red Fluorophore
(RCAMP, Jrgeco1A, dTomato)

Exc 555-570nm / Em 580-600nm

Opsins

ChR2
450 nm

N/A

Sequential

NpHR/Chrimson
Jaws
632 nm*

Simultaneous or Sequential

Simultaneous or Sequential

*560/590 on request


    Wavelength range 350 to 1100 nm
    Field of view Ø3 mm
    Objective NA 0.40
    Maximum number of sites*
    		 - 7x core 400 µm NA0.37
    - 9x core 400 µm NA0.37
    - 19x core 200 µm NA0.37
    LEDs Excitation Uniformity 80% over FOV
    Optogenetics Excitation Uniformity 65% over FOV
    Optogenetics Crosstalk between fibers >31 dB
    Optical fiber compatibility - Core diameter 200, or 400 µm
    - NA 0.37
    Spectral bandwidth - Excitation #1 : 405-410, 410-415, 421-433 or 433-445nm
    - Excitation #2 : 460-490 nm
    - Excitation #3 : 555-570 nm
    - Emission #1 : 500-540 nm
    - Emission #2 : 580-600 nm
    Optical filter attenuation > OD 5 outside band
    Optical fiber connector HD7, HD9 or HD19
    Dimensions 220mm x 220mm x 59mm
    SENSOR
    Type CMOS image sensor
    Resolution 1024x1024 px
    Pixel size

    5.86 µm x 5.86 µm
    Frame rate up to 60 Hz
    Computer interface USB 3.0
    * could be limited by patchcord manufacturing
    Drawing

    Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics
    User ManualFrEn

    Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics

    • The Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics (BFTO) enables multi-site measurements through High-density Fiber-optic Patch Cords (HDP). These patch cords are available in either square or hexagonal patterns of 7, 9 or 19 fibers connected to the sample port, allowing combined optogenetics and photometry recording.


      Build-in LEDs combine Isosbestic & Functional GCaMP excitations with Red Fluorophore excitation. Isosbestic and functional fluorescent signals are recorded with the same image sensor by interleaving the excitations. Both the LED driver and the CMOS detector are integrated within the system. The optogenetics stimulation pattern can vary in type, number and emitted wavelengths. For this purpose, a second similar high-density fiber optic patch cord is connected to the optogenetic port and fans out into separate connectors. Then, up to 19 independent optogenetic stimulation inputs can be connected depending on the needs of the experiment (one input per fiber connected to the sample port).
      The BFTO system with targeted optogenetics supports the following fluorophore and opsin combinations:

       


      		 Fluorophores

      Green Fluorophore 
      (GCAMP, dLight)

      Exc 460-490nm / Em 500-540nm

      Red Fluorophore
      (RCAMP, Jrgeco1A, dTomato)

      Exc 555-570nm / Em 580-600nm

      Opsins

      ChR2
      450 nm

      N/A

      Sequential

      NpHR/Chrimson
      Jaws
      632 nm*

      Simultaneous or Sequential

      Simultaneous or Sequential

      *560/590 on request


      • Wavelength range 350 to 1100 nm
        Field of view Ø3 mm
        Objective NA 0.40
        Maximum number of sites*
        		 - 7x core 400 µm NA0.37
        - 9x core 400 µm NA0.37
        - 19x core 200 µm NA0.37
        LEDs Excitation Uniformity 80% over FOV
        Optogenetics Excitation Uniformity 65% over FOV
        Optogenetics Crosstalk between fibers >31 dB
        Optical fiber compatibility - Core diameter 200, or 400 µm
        - NA 0.37
        Spectral bandwidth - Excitation #1 : 405-410, 410-415, 421-433 or 433-445nm
        - Excitation #2 : 460-490 nm
        - Excitation #3 : 555-570 nm
        - Emission #1 : 500-540 nm
        - Emission #2 : 580-600 nm
        Optical filter attenuation > OD 5 outside band
        Optical fiber connector HD7, HD9 or HD19
        Dimensions 220mm x 220mm x 59mm
        SENSOR
        Type CMOS image sensor
        Resolution 1024x1024 px
        Pixel size

        5.86 µm x 5.86 µm
        Frame rate up to 60 Hz
        Computer interface USB 3.0
        * could be limited by patchcord manufacturing
      • Drawing

        Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics
        User ManualFrEn

        Bundle-imaging Fiber Photometry Cube with Targeted Optogenetics