8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics (OBSOLETE)
8 ports Bundle-imaging Fluorescence Mini Cubes - GCaMP, Red Fluorophore, Blue and Red Optogenetics (OBSOLETE)
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Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.
- The excitation ports have integrated LED light sources; UV, blue and yellow excitations.
- The detector ports come with integrated CMOS sensors; green and red emission.
- Combines GCaMP's calcium dependant signal with the calcium independant (isosbestic) reference.
- Optogenetics port for blue 450 nm laser (ChR2) is connectorized with FC/PC receptacle.
- Optogenetics port for red 638 nm laser (Chrimson, Jaws) is connectorized with FC/PC receptacle.
- To accomodate large fiber bundles, an SMA fiber optic receptacle is used on the sample port.
- Each excitation source should be interleaved and synchronized with the camera acquisition
| Wavelength range | 350 to 1100 nm |
| Field of view | 2.5 mm |
| Objective NA | 0.40 |
|
Maximum number of sites * could be limited by patchcord manufacturing |
- 20x core 400 um NA0.37 - 60x core 200 um NA0.37 - 100x core 100 um NA0.37 |
| Excitation Uniformity | 10% over FOV |
| Optical fiber compatibility |
- Core diameter 200 or 400 µm - NA 0.37 to 0.57 |
| Spectral bandwidth GCaMP |
- Excitation : 400-410 nm & 460-490 nm - Emission : 500-550 nm |
| Spectral bandwidth Red Fluorophore |
- Excitation : 555-570 nm - Emission : 580-620 nm |
| Spectral bandwidth Optogenetics | - Blue : 433 - 456 nm (for 450 nm laser) - Red : 628 - 642 nm (for 638 nm laser) |
| Optical filter attenuation | > OD 5 outside band |
| Optical fiber connector | SMA |
| Dimensions | |
| SENSOR | |
| Type | CMOS Image sensor |
| Quantum Efficiency |
82% at 520 nm |
| Resolution |
1024 x 1024 px |
| Pixel Size |
5.86 µm x 5.86 µm |
| Analog gain |
0 to 72 dB |
| Frame rate |
up to 60 Hz |
| Computer interface | USB 3.0 |
| Power consumption | max 200 mA (supplied by USB) |
| LEDs | |
| Max Current | 500 mA |
| Output Power (fibre 200 µm) |
LED 400-410 nm = 4 - 400 µW |
| LED 460-490 nm = 5 - 1000 µW | |
| LED 555-570 nm = 2 - 150 µW | |
| Output Power (fibre 400 µm) |
LED 400-410 nm = 15 - 1500 µW |
| LED 460-490 nm = 20 - 4000 µW | |
| LED 555-570 nm = 8 - 600 µW | |
-
Performing fiber photometry on a large number of sites and animals can be inconvenient due to the large number of photoreceivers and patch cords required. By bundling the connective patch cords together and using a camera, it is possible to measure a great number of sites or animals at once. The Bundle-imaging Fluorescence Mini Cube is able to perform such measurements. This is done by imaging a fiber bundle onto a camera using a microscope objective. These otherwise function as any fluorescence mini cube.
- The excitation ports have integrated LED light sources; UV, blue and yellow excitations.
- The detector ports come with integrated CMOS sensors; green and red emission.
- Combines GCaMP's calcium dependant signal with the calcium independant (isosbestic) reference.
- Optogenetics port for blue 450 nm laser (ChR2) is connectorized with FC/PC receptacle.
- Optogenetics port for red 638 nm laser (Chrimson, Jaws) is connectorized with FC/PC receptacle.
- To accomodate large fiber bundles, an SMA fiber optic receptacle is used on the sample port.
- Each excitation source should be interleaved and synchronized with the camera acquisition
-
Wavelength range 350 to 1100 nm Field of view 2.5 mm Objective NA 0.40 Maximum number of sites
* could be limited by patchcord manufacturing- 20x core 400 um NA0.37
- 60x core 200 um NA0.37
- 100x core 100 um NA0.37Excitation Uniformity 10% over FOV Optical fiber compatibility - Core diameter 200 or 400 µm
- NA 0.37 to 0.57Spectral bandwidth GCaMP - Excitation : 400-410 nm & 460-490 nm
- Emission : 500-550 nmSpectral bandwidth Red Fluorophore - Excitation : 555-570 nm
- Emission : 580-620 nmSpectral bandwidth Optogenetics - Blue : 433 - 456 nm (for 450 nm laser)
- Red : 628 - 642 nm (for 638 nm laser)Optical filter attenuation > OD 5 outside band Optical fiber connector SMA Dimensions SENSOR Type CMOS Image sensor
Quantum Efficiency
82% at 520 nm Resolution
1024 x 1024 px
Pixel Size
5.86 µm x 5.86 µm
Analog gain
0 to 72 dB
Frame rate
up to 60 Hz
Computer interface USB 3.0
Power consumption max 200 mA (supplied by USB)
LEDs Max Current 500 mA Output Power
(fibre 200 µm)LED 400-410 nm = 4 - 400 µW
LED 460-490 nm = 5 - 1000 µW LED 555-570 nm = 2 - 150 µW Output Power
(fibre 400 µm)LED 400-410 nm = 15 - 1500 µW LED 460-490 nm = 20 - 4000 µW LED 555-570 nm = 8 - 600 µW